Scan and follow us
Learn more
Tools | Function | Product Offering |
---|---|---|
mCherry-EGFP-RAMP4 | ER-phagy | LV/Ad/AAV |
mCherry-RAMP4 | ||
ssmRFP1-EGFP-KDEL |
1)mCherry-EGFP-RAMP4 and ssmRFP1-EGFP-KDEL In the cytoplasm, co-expression of red and green fluorescent proteins leads to the generation of yellow punctas. Upon induction of ER-phagy, the acidic environment within lysosomes quenches the green fluorescence, leaving only the red fluorescence intact. The quantification of red fluorescent punctas enables monitoring of ER-phagy.
PMID:30143524(mCherry-EGFP-RAMP4工具)
PMID: 31006538(ssmRFP1-EGFP-KDEL工具)
Moreover, in the lysosomes, cleavage occurs between mRFP1 and EGFP in the ssmRFP1-EGFP-KDEL tool. The determination of mRFP1/mRFP1-EGFP ratio through Western Blot analysis provides an indication for ER-phagy.
PMID: 31006538(ssmRFP1-EGFP-KDEL工具)
2)mCherry-RAMP4:
In the cytoplasm, expression of fusion protein mCherry-RAMP4 takes place; upon initiation of ER-phagy, hydrolytic enzymes cleave this fusion protein to release the mCherry fluorescence protein. Calculation of mCherry/mCherry-RAMP4 band intensity ratio from Western Blot results serves as an indicator for ER-phagy
联系我们
返回顶部