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Adenovirus Packaging

Recombinant adenovirus (AdV) is a widely used replication-defective adenoviral vector system in gene therapy, basic life science research, and other fields. The commonly used adenoviral vectors are based on human adenovirus type 5 (Ad5), which has a linear double-stranded DNA genome of 36 kb. Adenoviruses can enter cells by binding their fibers to cell surface receptors, followed by internalization into endosomes and subsequent transfer to the cytoplasm and nucleus. With the assistance of cellular transcription and translation machinery, viral replication and assembly are initiated. A complete viral life cycle leads to cell death, resulting in the release of viral particles.

Adenoviruses have the following characteristics

Wide range of infection

Adenovirus can be used to infect ordinary cell lines, suspended cells, primary cells and so on

High infection efficiency

The titer of adenovirus can be as high as 10¹¹PFU/ml, and the infection efficiency is high, which can infect suspension cells and primary cells.

Large carrier capacity

The vector capacity of adenovirus is large, up to 8kb.

Hanbio provides adenovirus-related services

1. Conventional gene overexpression/interfering Adenovirus pacakging

2.MiRNAsponge/antago adenovirus

3.CircRNA /LncRNA overexpression and interference adenovirus

4.CRISPR/Cas9 denovirus customization

5.Adenovirus purification services

6.Adenovirus stock products

Hanbio provides products

Products	Titer	Application
Common adenovirus	10¹⁰PFU/ml or 10¹¹PFU/ml	Suitable for common cell line infection
Suspended cell specific adenovirus（Ads）	10¹⁰PFU/ml or 10¹¹PFU/ml	Specially designed for suspended cells, compatible with normal and primary cells, with higher efficiency

Our Advantage

1.Focus on providing virus packaging service for more than twelve years

2.10,000+customers cooperate with Hanhen in a year

3.Tens thousands of SCI journal citations, including CELL, Nature Medicine and other top journals

4.ISO9001 certified quality system, strict virus purification process

5.The improved customer service system, technical support, project follow-up, and after-sales service have garnered unanimous praise from customers, guaranteeing a hassle-free ordering experience for you.

Adenovirus Vecters

NO.	Regulation	Vector Name	Prokaryotic Antibiotic	Eukaryotic Antibiotic	Fluorescent Label	Promoter
Adeasy014	OE	pAdEasy-EF1-MCS-CMV-EGFP	Kanamycin	not have	EGFP	EF1
Adeasy015	OE	pAdEasy-EF1-MCS-CMV-mCherry	Kanamycin	not have	mCherry	EF1
Adeasy018	OE	pAdEasy-CMV	Kanamycin	not have	not have	CMV
Adeasy022	OE	pAdEasy-CMV-3flag	Kanamycin	not have	not have	CMV
Adeasy023	OE	pAdEasy-EF1-MCS-3flag-CMV-EGFP	Kanamycin	not have	EGFP	EF1
Adeasy024	OE	pAdEasy-EF1-MCS-CMV-LUC	Kanamycin	not have	Luciferase	EF1
Adeasy027	OE	pAdEasy-EF1-MCS-CMV-eRFP	Kanamycin	not have	eRFP	EF1
Adeasy030	OE	pAdEasy-EF1-MCS-3flag-CMV-mCherry	Kanamycin	not have	mCherry	EF1
Adeasy032	OE	pAdeasy-EF1-CircRNA-CMV-EGFP	Kanamycin	not have	EGFP	EF1
Adeasy019	RNAi	pAdEasy-U6	Kanamycin	not have	not have	U6
Adeasy020	RNAi	pAdEasy-U6-CMV-EGFP	Kanamycin	not have	EGFP	U6
Adeasy021	RNAi	pAdEasy-U6-CMV-mCherry	Kanamycin	not have	mCherry	U6
Adeasy028	RNAi	pAdEasy-U6-CMV-eRFP	Kanamycin	not have	eRFP	U6
Adeasy026	Cas9/gRNA	pAdEasy-U6-CMV-Cas9-2A-eRFP	Kanamycin	not have	eRFP	U6
Adeasy043	Cas9/gRNA	pAdEasy-U6-scaffold-CMV-Cas9-T2A-ZsGreen	Kanamycin	not have	ZsGreen	U6

Example of adenovirus infection

腺Adenovirus infection of adherent cells

HCT116 and HT29 transfected with mRFP–GFP–LC3 adenovirus,hanbio’s customer published in CELL in 2017

Adenovirus infection of primary cells

Adenoviruses used in animal experiments

The titer of adenovirus purification can reach 10¹¹PFU/ml, which is used for animal experiments

Mice were injected subconjunctival with purified adenovirus Ad-SIRT1 and control AdGFP,PFU=10¹¹/ml，3 μl/single eye, GFP was observed 48 hours later

Excerpts from customer published literature

Fusobacterium nucleatum Promotes Chemoresistance to Colorectal Cancer by Modulating Autophagy

（Journal:CELL，IF=41.582，Hospital, School of Medicine, Shanghai Jiao Tong University）

Local hyperthermia therapy induces browning of white fat and treats obesity

（Journal:CELL，IF=41.582，East China Normal University）

TRIB3 Promotes APL Progression through Stabilization of the Oncoprotein PML-RARa and Inhibition of p53-Mediated Senescence

（Journal:Cancer Cell，IF=31.743，Institute of Materia Medica）

An ALOX12–12-HETE–GPR31 signaling axis is a key mediator of hepatic ischemia–reperfusion injury

（Journal:NATURE MEDICINE，IF=30.641,Wuhan University）

WWC proteins mediate LATS1/2 activation by Hippo kinases and imply a tumor suppression strategy

（Journal:MOLECULAR CELL，IF=17.970，Children’s Hospital of Fudan University）

E3 ubiquitin ligase RNF5 protects against hepatic ischemia reperfusion injury by mediating PGAM5 ubiquitination

（Journal:HEPATOLOGY，IF=17.425，The First Affiliated Hospital of Zhengzhou University）

SIRT3 Acts As a Negative Regulator of Autophagy Dictating Hepatocyte Susceptibility to Lipotoxicity

（Journal:HEPATOLOGY，IF=17.425，Zhejiang University）

atg7-Based Autophagy Activation Reverses Doxorubicin-Induced Cardiotoxicity

（Journal:Circulation Research，IF=17.367，Beijing University of Chinese Medicine）

Protective effect of HINT2 on mitochondrial function via repressing MCU complex activation attenuates cardiac microvascular ischemia–reperfusion injury

（Journal：Basic Research in Cardiology，IF=17.165，Zhongshan Hospital, Fudan University）

AKAP1 Deficiency Attenuates Diet-Induced Obesity and Insulin Resistance by Promoting Fatty Acid Oxidation and Thermogenesis in Brown Adipocytes

（Journal：Advanced Science，IF=16.806，Fourth Military Medical University）