CCK-8试剂盒

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CCK-8 kit

Cell proliferation is the basis of organism growth, development, reproduction and heredity, and is an important life characteristic of organism. Cells usually proliferate by dividing. Cytotoxic is a simple cell-killing event caused by cells or chemicals, which is not dependent on apoptosis or necrosis.

Cell Counting Kit-8, referred to as CCK-8 Kit, an alternative method of MTT assay, is a rapid and highly sensitive detection kit based on WST (water-soluble tetrazolium salt, chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfobenzene)-2H-tetrazolium monosodium salt) widely used in cell proliferation and cytotoxicity. It can be used for drug screening, cell proliferation assay, cytotoxicity assay, tumor drug sensitivity, and other tests.

Product packaging and storage conditions：

Dry and keep in the dark at 4℃, valid for one year (recommended). -20℃ dry and stored in the dark, valid for two years.

Item No.	Product name	Number of use	Volume
HB-CCK-8-500	CCK-8 cell proliferation detection kit	500 tests	5mL
HB-CCK-8-1000	CCK-8 cell proliferation detection kit	1000 tests	10mL
HB-CCK-8-3000	CCK-8 cell proliferation detection kit	3000 tests	30mL
HB-CCK-8-10000	CCK-8 cell proliferation detection kit	10000 tests	100mL

Experimental principle:

CCK-8 cell proliferation detection kit is a rapid sensitivity kit based on WST-8 (chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfonyl benzene)), which is widely used in cell proliferation and cytotoxicity detection. The WST-8 is an upgrade of MTT and works as follows: In the presence of electron-coupled reagents, WST-8 can be reduced by the mitochondrial dehydrogenase to produce the highly water-soluble orange-yellow formazan (Figure 1-2), the color of which is proportional to the proliferation of the number of surviving cells and inversely proportional to cytotoxicity. OD values measured at 450 nm wavelength by enzyme-labeled apparatus can indirectly reflect the number of living cells. CCK-8 method is widely used, such as drug screening, cell proliferation assay, cytotoxicity assay, tumor drug susceptibility assay, and biological factor activity detection.

Advantages of CCK-8 method:

Table 1 The advantages of CCK-8 method compared with other cell proliferation / toxicity detection methods

Comparison item	MTT assay	XTT assay	WST-1 assay	CCK-8 assay
Water solubility of formzan product	Poor (Need to add organic solvent to dissolve and then test)	Good	Good	Good
Shape of product	Powder	2 bottles of solution	solution	1 bottle of solution
Method of Use	The powder was prepared into a solution before use.	Preparation as needed.	Out-of-the-box.	Out-of-the-box.
Sensitivity of detection	High	Very high	Very high	High
Detection time	Long	Short	Short	Shortest
Wavelength of detection	500-600nm	420-480nm	420-480nm	430-490nm
Cytotoxic	High, complete loss of cell morphology	High, complete loss of cell morphology	High, complete loss of cell morphology	High, complete loss of cell morphology
Stability of reagents	General	Poor	General	Very good
Batch sample testing	Yes	Very suitable	Very suitable	Very suitable
Convenience	General	Convenient	Convenient	Very convenient

Note:

1. Phenol red and serum did not interfere with the detection of CCK-8 method;

2. The cytotoxicity of this kit is very low, so after adding WST-8 for color development, the best assay time can be found by repeatedly reading with the microplate reader at different times.