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HanBio provides luciferase and dual luciferase detection kits to help you operate experiments easily and efficiently.
Luciferase(firefly)Reporter Assay Kit(100 rxns) | |
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Luciferase Reaction Buffer | 10mL |
Luciferase Reaction Substrate(Lyophilized) | 1 vial |
Lysis Buffer(5X) | 10mL |
Firefly luciferase (fluc) can catalyze the bioluminescence reaction of luciferin. The reaction process is shown in the following figure
The assay kit used fluorescein as a substrate to detect the activity of firefly reporter gene. This method has the advantages of simple operation, fast detection and high sensitivity.
Dual-Luciferase Reporter Assay Kit(100 rxns) | |
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Luciferase Reaction Buffer | 10mL |
Luciferase Reaction Substrate(Lyophilized) | 1 vial |
Luciferase Reaction Buffer II | 10mL |
Luciferase Reaction Substrate II(125X) | 80μL |
Lysis Buffer(5X) | 10mL |
Firefly luciferase (fluc) and renilla luciferase (renilla luciferase, rluc) can catalyze the bioluminescence reaction of luciferin and coelenterazine, respectively, as shown in the following figure.
In the dual-luciferase Reaction, the fLuc signal was detected by Luciferase Reaction Reagent I. Subsequently, Luciferase Reaction Reagent II was added directly. The signal of fLuc was quenched and the rLuc signal was generated at the same time. This method has the advantages of simple operation, fast detection and high sensitivity.
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