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LncRNA Service

Long non-coding RNA（lncRNA）is a class of long non-coding RNA molecule with a length of more than 200nt,which is a by-product of RNA polymerase II transcription. It can regulate gene expression at various levels (epigenetic regulation, transcriptional regulation and post-transcriptional regulation, etc.). It is a research hotspot of genetics.

Research scheme of lncRNA system based on ceRNA regulatory mechanism of Hanbio：

1）To verify the expression level and intracellular sublocalization of lncRNA;

2）Predict the miRNAs that lncRNAs may bind to through network database;

3）Informatic screening of lncRNA-bound miRNAs and further screening and validation by luciferase assay;

4） miRNA target gene prediction and functional enrichment analysis;

5）Screening, verification and related functional studies of miRNA target genes;

6）Verification of lncRNA-miRNA-mRNA regulatory network.

Hanbio validation services advantages on lncRNA and target gene competitive binding microRNA :

1）Professional software was used to predict the binding sites of miRNA, lncRNA and target genes;

2）psiCHECK plasmid unique to Promega Company was adopted;

3）The Luciferase dual fluorescence reporting system can obtain a wide linear quantitative range and extremely high sensitivity;

4）The instantaneous transfection technique is simple, rapid and short period, and is suitable for most cell types.

Hanbio lncRNA service：

1）lncRNA overexpression and interference plasmid construction;

2）lncRNA overexpression and interference virus packaging;

3）Stable cell lines construction service based on lentivirus technology;

4）Verification service of lncRNA and target gene competitively bind with microRNA ;

5）Service of ceRNA mechanism research.