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1)To verify the expression level and intracellular sublocalization of lncRNA;
2)Predict the miRNAs that lncRNAs may bind to through network database;
3)Informatic screening of lncRNA-bound miRNAs and further screening and validation by luciferase assay;
4) miRNA target gene prediction and functional enrichment analysis;
5)Screening, verification and related functional studies of miRNA target genes;
6)Verification of lncRNA-miRNA-mRNA regulatory network.
1)Professional software was used to predict the binding sites of miRNA, lncRNA and target genes;
2)psiCHECK plasmid unique to Promega Company was adopted;
3)The Luciferase dual fluorescence reporting system can obtain a wide linear quantitative range and extremely high sensitivity;
4)The instantaneous transfection technique is simple, rapid and short period, and is suitable for most cell types.
1)lncRNA overexpression and interference plasmid construction;
2)lncRNA overexpression and interference virus packaging;
3)Stable cell lines construction service based on lentivirus technology;
4)Verification service of lncRNA and target gene competitively bind with microRNA ;
5)Service of ceRNA mechanism research.
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