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Home > Product > Luciferase Assay

Reporter Gene Plasmid

HanBio offers a variety of engineered cell lines and pre-constructed reporter vectors for analyzing specific biological pathways. For example, luciferase reporter vectors for analyzing cytokine regulation, activation of signaling pathway and cytotoxicity including heat shock, hypoxia and DNA damage.

The reporter gene plasmids provided by Hanbio :

Plasmid Name	Fluorescent type	eukaryotic resistance	note	Functional Description
pHBLuc-FOPFlash	Luciferase	none	FOPFlash, as a internal reference plasmid of β-catenin pathway	This eukaryotic expression vector contains mutated TCF/LEF binding sites and can be used as an internal reference plasmid for β-catenin pathway assays.
pHBLuc-TOPFlash	Luciferase	none	FOPFlash, as a internal reference plasmid of β-catenin pathway	This eukaryotic expression vector contains mutated TCF/LEF binding sites and can be used as an internal reference plasmid for β-catenin pathway assays.。
pHBLuc-CRE	Luciferase	Hygromycin	CRE for detecting the activation of CRE in the cAMP/PKA pathway	CREB (cAMP-response element binding protein) is an intracellular nuclear regulator that achieves function of regulating transcription through autophosphorylation, and affects the functions related to development of fetal T-cell, cell survival, and the molecular neural mechanisms of learning and memory. This eukaryotic expression vector has a CRE binding site inserted in MCS, which can detect the activation level of cAMP/PKA signaling pathway with high sensitivity.
pHBLuc-NFAT-RE	Luciferase	Hygromycin	NFAT-RE for detecting the activation of NFAT in the PKC/Ca++ pathway	The NFAT (Nuclear factor of activated T-cells) family of transcription factors plays a key role in the transcription of cytokine genes and a number of other genes critical for the immune response. This eukaryotic expression vector has an NFAT binding site inserted in MCS, which can detect the activation level of NFAT with high sensitivity.
pHBLuc-NF-kB-RE	Luciferase	Hygromycin	NF-kB-RE for detecting activation of NF-κB	NF-κB gene has an important role in inflammatory response, immune response and inhibition of apoptosis. This eukaryotic expression vector has NF-κB binding site inserted in MCS, which can detect the activation level of NF-κB with high sensitivity.
pHBLuc-SRE	Luciferase	Hygromycin	SRE for detecting activation of MAPK/ERK signaling pathway	This eukaryotic expression vector has an SRE binding site inserted in MCS, which can detect the activation level of MAPK/ERK signaling pathway with high sensitivity.
pHBLuc-ARE	Luciferase	Hygromycin	ARE for detecting the activation of ARE	ARE (antioxidant response element) binds to Nrf2 and NRF1 transcription factors and regulates related genes involved in the protection of cells against oxidative damage. The eukaryotic expression vector is inserted ARE binding site in MCS, which can detect the antioxidant level or oxidative stress status of cells with high sensitivity.
pHBLuc-p53RE	Luciferase	Hygromycin	p53RE for detecting activation of p53	p53 is an important anticancer gene making cancer cells commit suicide and preventing carcinogenesis, among other functions. This eukaryotic expression vector has a p53 binding site inserted in MCS, which can efficiently detect the activation level of p53.
pHBLuc-ATF6RE	Luciferase	Hygromycin	ATF6RE for detecting activation of ATF6	ATF6 (Activating Transcription Factor 6) is a transcription factor that plays a role in endoplasmic reticulum stress by activating unfolded proteins in response to target genes. This eukaryotic expression vector has an ATF6 binding site inserted in MCS, which can detect the activation level of ATF6 with high sensitivity.
pHBLuc-MRE	Luciferase	Hygromycin	MRE for detecting activation of MTF	MTF1 (Metal regulatory transcription factor 1) plays a key role in the transcriptional regulation of metallothioneins and genes involved in metal homeostasis and cellular stress response in vivo. This eukaryotic expression vector has inserted MTF1 binding site in MCS, which can efficiently detect the activation level of MTF1.
pHBLuc-HSE	Luciferase	Hygromycin	HSE for detecting the activation of HSF in the test	HSF (heat shock transcription factors) mainly lies in the control of the dynamic balance of heat stress response. This eukaryotic expression vector inserted HSF binding site in MCS, which can detect the activation level of HSF with high sensitivity.
pHBLuc-HRE	Luciferase	Hygromycin	HRE for detecting the activation of HIF1	HIF-1 (hypoxia-inducible factor-1) is a key protein related to oxygen homeostasis and has a significant role in cancer development, as well as in cardiovascular disease. This eukaryotic expression vector has an HRE binding site inserted in MCS, which can detect the activation level of HIF1 with high sensitivity.
pHBLuc-XRE	Luciferase	Hygromycin	XRE for detecting the activation of AHR	AhR (Aryl hydrocarbon receptor ) is a nuclear receptor transcription factor widely involved in multiple intracellular signaling pathways. This eukaryotic expression vector has an AhR binding site inserted in MCS, which can detect the activation level of AhR with high sensitivity.
pHBLuc-APIRE	Luciferase	Hygromycin	APIRE for detecting the activation of AP-1	AP-1 (activating protein-1) is a class of nuclear transcription factors involved in transformation, differentiation, apoptosis and other biological functions. This eukaryotic expression vector has an AP-1 binding site inserted in MCS, which can detect the activation level of AP-1 with high sensitivity.
pHBLuc-ISRE	Luciferase	Hygromycin	ISRE for detecting the activation of STAT1/STAT2	STATs are a class of important nuclear transcription factors, including STAT1-STAT4, STAT5a, STAT5b and STAT6, a total of seven members, which are closely related to cell proliferation, differentiation and apoptosis, among which STAT1 and STAT2 are involved in apoptosis control. This eukaryotic expression vector has STAT1/STAT2 binding sites inserted in MCS, which can detect the activation level of STAT1/STAT2 with high sensitivity.
pHBLuc-SIE	Luciferase	Hygromycin	SIE for detecting the activation of STAT	SIE for detecting the activation of STAT3	After activating STAT3, STAT3 dimers and STAT1/STAT3 heterodimers are formed, which interact with SIS inducible element (SIE) reaction elements to regulate cell growth and apoptosis. The eukaryotic expression vector inserted STAT3 binding site in its Multiple cloning site, which can detect the activation level of STAT3 with high sensitivity.
pHBLuc-SBE	Luciferase	Hygromycin	SBE for detecting the activation of SMAD	The SMAD (drosophila mothers against decapentaplegic ) family of proteins plays a critical role in transducing TGF-β signaling from cell surface receptors to the nucleus, and different Smads mediate the signaling of different TGF-β family members. This eukaryotic expression vector has inserted a SMAD binding site in MCS, which can detect the activation level of SMAD with high sensitivity.
pHBLuc-TCF-LEF	Luciferase	Hygromycin	TCF-LEF for detecting the activation of TCF/LEF in the Wnt pathway	This eukaryotic expression vector has multiple TCF/LEF1 binding sites inserted in MCS, which can detect the activation level of TCF/LEF1 in the Wnt signaling pathway with high sensitivity.
pHBLuc-STAT5 RE	Luciferase	Hygromycin	STAT5 RE for detecting the activation of Jak/Stat signaling pathway	Stat5 is an important member of the signal transduction and activator of transcription (Stat) family of proteins involved in the regulation of the Jak/Stat signaling pathway. This eukaryotic expression vector inserted a STAT5 binding site in MCS, which can detect the activation level of STAT5 in Jak/Stat signaling pathway with high sensitivity.